Abstract: Co-culture technology as a new fermentation strategy can greatly increase target product yields, which is promising for the application of biochemical, pharmaceutical and food industry. In this paper, the flat transparent circle method was firstly used for screening naringinase production strains, and then the effects of various fermentation parameters on naringinase liquid fermentation process via fungi co-culture were investigated. The results show that A. niger uv-11 and Rhizopus 3201 are the suitable co-culture strains, and naringinase activity reaches to 733.19 U·mL^-1 when the inoculation time interval is 36 h, the inoculation volume is 10%, the inoculation ratio is 2:1, the initial pH of broth is 5.5 and the total fermentation time is 72 h. This activity is 2.22 times higher than that under not optimized condition. This study provides basic data for exploring naringinase co-culture fermentation process and increasing naringinase activity, which has great significance for promoting naringinase industrialization process.