竹叶发酵液的美白、增强皮肤屏障和抗炎护肤功效研究

Effects of bamboo leaf fermentation broth on the whitening, skin barrier strengthening and anti-inflammatory skincare functions

摘要: 为研究竹叶发酵液(FBL)的护肤功效，选取角质形成细胞(HaCaT)和黑色素瘤细胞(B16)作为细胞模型，利用细胞计数试剂(CCK-8)评估竹叶发酵液的细胞毒性；并通过检测黑色素含量、十二烷基硫酸钠(SLS)损伤模型下丝聚蛋白和炎症因子的基因表达，分别考察FBL的美白、增强皮肤屏障和抗炎的护肤功效。结果表明，FBL对上述细胞均没有毒性，同时能够显著降低B16细胞内黑色素的含量(显著性p < 0.001)，并能促进丝聚蛋白的基因表达(p < 0.001)、抑制肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)的基因表达(p < 0.001，p < 0.01)。因此，FBL具有潜在的护肤功效。

Abstract: HaCaT cells and B16 cells were applied to establish evaluation models to study the effects of bamboo leaf fermentation broth (FBL) on skincare functions. CCK-8 test was performed to evaluate cell toxicity of FBL. The whitening, skin barrier strengthening and anti-inflammatory effects of FBL were determined by melanin content assay, gene expression assay of filaggrin and inflammatory factors under sodium lauryl sulfate injury model, respectively. The results show that FBL has no toxicity to HaCaT and B16 cells. FBL can significantly reduce melanin contents in B16 cells (p < 0.001), promote filaggrin gene expression (p < 0.001) and inhibit the expression of TNF-α and IL-6 (p < 0.001, p < 0.01). In conclusion, FBL has potential skincare effects.