Abstract: Composite microspheres were prepared by an oil-water emulsion and evaporation method, which were then sintered to prepare microsphere scaffolds. The compressive strength, porosity and pore connection rate of the scaffold can be modified by adjusting β-TCP amount, which results a scaffold with compressive strength in the range of cancellous bone, with porosity and pore connection rate large enough to allow cell grow. The surface smoothness decreases with the addition of 0.1, 0.2, 0.3 and 0.4 g β-TCP in PLGA. The adhesion degree decreases and the porosity and average diameter increase with the increase of β-TCP after sintering at 70℃ for 1 h. The average pore diameter is 84.34 μm when adding 0.3 g β-TCP, which is larger than the least diameter (80 μm) for cell grow in scaffolds. Compressive strength increases first and then decreases, which shows the highest value of (6.05±0.74) MPa when adds 0.2 g β-TCP. However, the connection between microspheres decreases with high β-TCP, which results in the decrease of compressive strengths of the scaffolds. Cytocompatibility can be enhanced with the increase of β-TCP. Therefore, an optimum β-TCP amount was found (0.3 g) to prepare microsphere scaffolds with best comprehensive properties.